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pVAW409R1
pVAW409R1
規(guī)格:
貨期:
編號:B240714
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pVAW409R1
商品貨號 B240714
Designations pVAW409R1
Species Homo sapiens, human
Vector
Construct size (kb): 13.69999980926514
Insert
DNA: genomic
Insert lengths(kb): 11.0
Gene product: DNA Segment, single copy [D17S122]
Alleles: A1, A1, A2, A3, B1, A2, B2
Insert Size (kb) 11.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.4, 4.3; EcoRI--11.0, 2.7; BamHI--6.0, 3.2, 2.4, 2.1; PstI--2.5 (doublet), 2.4, 2.0, 1.1 (doublet), 0.7, 0.6, 0.4; SalI--13.7.
This is a subclone of VAW409 (p07486).
This probe may contain repetitive sequences.
The 2.8/2.7 kb constant bands are the same polymorphism detected by pVAW409R3 (ATCC 61476, 61477) but hybridization is light and the 1.9 kb constant band blocks reading the 1.9 kb allele. The 2.7 band obscures reading the A2 allele.
The combined heterozygosity of both MspI polymorphisms at D17S122 detected by pVAW409R1 (ATCC 61474, 61475) and pVAW409R3 (ATCC 61476, 61477) is 72%.
References

Wright EC, et al. A genetic map of human chromosome 17p. Genomics 7: 103-109, 1990. PubMed: 2335351

Lebo RV, et al. Multicolor in situ hybridization and linkage analysis order Charcot-Marie-Tooth type 1 (CMT1A) gene-region markers. Am. J. Hum. Genet. 50: 42-55, 1992. PubMed: 1729894

Lupski JR, et al. DNA duplication associated with Charcot-Marie-Tooth disease type 1A. Cell 66: 219-232, 1991. PubMed: 1677316

David F Barker, personal communication

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