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produces protein estrogen receptor

Construct size (kb): 3.759999990463257

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIC20H
Intact vector size: 2.722
Type of vector: plasmid
Vector end: SmaI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI
EcoRV BglII XhoI NruI
Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI
EcoRV XbaI BglII XhoI SacI NruI HindIII
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L08912

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ESR
Genomic copy number: unique
Gene name: estrogen receptor
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 q25.1
Type of DNA: cDNA
Insert 5' end: NaeI
Insert 3' end: EcoRI
Insert size (kb): 1.06
Cross references: DNA Seq. Acc.: M12674

Insert lengths(kb): 1.059999942779541

Gene product: estrogen receptor [ESR]

Alleles: A1, A2

Target Gene: estrogen receptor

Restriction digests of the clone give the following sizes (kb): EcoRI--3.9; BglII--3.3, 0.54; HindIII--2.6, 0.88, 0.33; PstI--3.2, 0.64; XbaI--3.4, 0.43; HindIII/SstI--2.7, 0.76, 0.34.

The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.55; HindIII--0.30; NcoI--0.46; PstI--0.60, 0.77; StuI--0.76; XbaI--0.41.

Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies.

It binds estrogens and antiestrogens.

Contains coding sequence for amino acids 241-595 including the stop codon.

The recombinant fusion protein contains an additional 18 N-terminal amino acids encoded by the vector polylinker.