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Size (kb): 5.1960000991821290

Vector: pSNAG (phagemid)

Promoters: Promoter CMV

Construction: pSCTEV, pSuperVSV

Marker(s):ampR

Construct size (kb): 5.196000099182129

Features: marker(s): ampR
other: Gal4p DNA-binding domain (DBD)
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: SfuI...XbaI
terminator: SV40
epitope tag: VSV-G

expression vector

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2.

The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector.

Temperature: 37.0°C

component of:ATCC 87677