一级特黄aaa大片在线观看,国产看免费视频,一级毛片,免费无码婬片aaa直播,久久久久国产一区二区三区,性色生活片久久无少妇一级婬片免费放,国产寡妇高潮一级毛片免费看

Size (kb): 4.320

Vector: pFN476 (plasmid)

Promoters: Promoter lac

Construction: pGEM3Z, pKA101-6

Marker(s):ampR

Construct size (kb): 4.320

Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR

expression vector T7 promoter

vector permitting RNA synthesis in vitro

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

vector with low copy number

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1.

Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.

Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell.

Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts.

T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303).

Temperature: 37.0°C